quantitect or miscript reverse transcription kit Search Results


95
PCR Biosystems Ltd ultrascript 2 0 cdna synthesis kit
Ultrascript 2 0 Cdna Synthesis Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher taqman reverse transcription reagent kit
Taqman Reverse Transcription Reagent Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Qiagen sybr green pcr kit
Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tiangen biotech co quantscript reverse transcription rt kit
Quantscript Reverse Transcription Rt Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PCR Biosystems Ltd qpcrbio cdna synthesis kit
Qpcrbio Cdna Synthesis Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
PCR Biosystems Ltd pcrbio 1 step
Pcrbio 1 Step, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen quantinova reverse transcription kit
Quantinova Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen quant inova reverse transcription kit
Quant Inova Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen reverse transcriptase kit
Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen omniscript reverse transcription kit
Omniscript Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Qiagen superscript iii reverse transcriptase kit
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93
Cusabio human telomerase reverse transcriptase levels
Figure 3. GREM1 overexpression inhibited ADSC senescence. (a) SA-β-gal staining showed fewer positive cells in GREM1 over- expressing ADSCs compared to vector control. Scale bar: 100 μm. (b) A quantitative analysis of SA-β-gal positive cells. (c) The relative <t>telomerase</t> activity was measured by the telomerase ELISA kit. (d, e) Real-time RT-PCR results showed that GREM1 overexpression decreased the expressions of P16 (d) and P53 (e). The control gene was GAPDH. The statistical significance was evaluated by Student’s t-test. The error bar was indicated by SD. (n = 3). *p ≤0.05.
Human Telomerase Reverse Transcriptase Levels, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3. GREM1 overexpression inhibited ADSC senescence. (a) SA-β-gal staining showed fewer positive cells in GREM1 over- expressing ADSCs compared to vector control. Scale bar: 100 μm. (b) A quantitative analysis of SA-β-gal positive cells. (c) The relative telomerase activity was measured by the telomerase ELISA kit. (d, e) Real-time RT-PCR results showed that GREM1 overexpression decreased the expressions of P16 (d) and P53 (e). The control gene was GAPDH. The statistical significance was evaluated by Student’s t-test. The error bar was indicated by SD. (n = 3). *p ≤0.05.

Journal: Connective tissue research

Article Title: GREM1 inhibits osteogenic differentiation, senescence and BMP transcription of adipose-derived stem cells.

doi: 10.1080/03008207.2020.1736054

Figure Lengend Snippet: Figure 3. GREM1 overexpression inhibited ADSC senescence. (a) SA-β-gal staining showed fewer positive cells in GREM1 over- expressing ADSCs compared to vector control. Scale bar: 100 μm. (b) A quantitative analysis of SA-β-gal positive cells. (c) The relative telomerase activity was measured by the telomerase ELISA kit. (d, e) Real-time RT-PCR results showed that GREM1 overexpression decreased the expressions of P16 (d) and P53 (e). The control gene was GAPDH. The statistical significance was evaluated by Student’s t-test. The error bar was indicated by SD. (n = 3). *p ≤0.05.

Article Snippet: We measured the human telomerase reverse transcriptase levels in the cells by ELISA following the manufacturer’s protocol (Human Telomerase Reverse Transcriptase, TERT ELISA Kit, Cusabio, Wuhan, China).

Techniques: Over Expression, Staining, Expressing, Plasmid Preparation, Control, Activity Assay, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

Figure 4. GREM1 knockdown accelerated ADSC senescence. (a) SA-β-gal staining showed an increased number of positive cells in the ADSC-GREM1sh group compared to the control group. Scale bar: 100 μm. (b) The quantitative analysis of SA-β-gal positive cells. (c) Telomerase activity assay results showed that GREM1 knockdown significantly decreased ADSCs telomerase activity. (d, e) Real- time RT-PCR results showed that GREM1 knockdown upregulated the expressions of P16 (d) and P53 (e). The control gene was GAPDH. The statistical significance was evaluated by Student’s t-test. The error bar was indicated by SD. (n = 3). *p ≤0.05.

Journal: Connective tissue research

Article Title: GREM1 inhibits osteogenic differentiation, senescence and BMP transcription of adipose-derived stem cells.

doi: 10.1080/03008207.2020.1736054

Figure Lengend Snippet: Figure 4. GREM1 knockdown accelerated ADSC senescence. (a) SA-β-gal staining showed an increased number of positive cells in the ADSC-GREM1sh group compared to the control group. Scale bar: 100 μm. (b) The quantitative analysis of SA-β-gal positive cells. (c) Telomerase activity assay results showed that GREM1 knockdown significantly decreased ADSCs telomerase activity. (d, e) Real- time RT-PCR results showed that GREM1 knockdown upregulated the expressions of P16 (d) and P53 (e). The control gene was GAPDH. The statistical significance was evaluated by Student’s t-test. The error bar was indicated by SD. (n = 3). *p ≤0.05.

Article Snippet: We measured the human telomerase reverse transcriptase levels in the cells by ELISA following the manufacturer’s protocol (Human Telomerase Reverse Transcriptase, TERT ELISA Kit, Cusabio, Wuhan, China).

Techniques: Knockdown, Staining, Control, Telomerase Activity Assay, Activity Assay, Quantitative RT-PCR